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Analysis of essential genes in Clostridioides difficile by CRISPRi and Tn-seq

ABSTRACT Essential genes are interesting in their own right and as potential antibiotic targets. To date, only one report has identified essential genes on a genome-wide scale in Clostridioides difficile, a problematic pathogen for which treatment options are limited. That foundational study used la...

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Autori principali: Maia E. Alberts, Micaila P. Kurtz, Ute Müh, Jonathon P. Bernardi, Kevin W. Bollinger, Horia A. Dobrila, Leonard Duncan, Hannah M. Laster, Andres J. Orea, Anthony G. Pannullo, Juan G. Rivera-Rosado, Facundo V. Torres, Craig D. Ellermeier, David S. Weiss
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2025-10-01
Serie:Journal of Bacteriology
Soggetti:
CRISPRi
Tn-seq
Accesso online:https://journals.asm.org/doi/10.1128/jb.00220-25
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