Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED

Simultaneous super-resolution imaging of multiple fluorophores remains a major challenge in STimulated Emission Depletion (STED) microscopy due to spectral overlap of STED-compatible fluorophores. The combination of STED microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) offers a powerfu...

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Detalhes bibliográficos

Principais autores:	Deschênes Andréanne, Ollier Antoine, Lafontaine Marie, Michaud-Gagnon Albert, Santiague Jeffrey-Gabriel Steavan, Bilodeau Anthony, Gagné Christian, De Koninck Paul, Lavoie-Cardinal Flavie
Formato:	Artigo
Idioma:	Inglês
Publicado em:	De Gruyter 2025-12-01
Colecção:	Methods in Microscopy
Assuntos:	sted; split-sted multicolor flim phasors
Acesso em linha:	https://doi.org/10.1515/mim-2025-0026
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