Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED
Simultaneous super-resolution imaging of multiple fluorophores remains a major challenge in STimulated Emission Depletion (STED) microscopy due to spectral overlap of STED-compatible fluorophores. The combination of STED microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) offers a powerfu...
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| Glavni autori: | , , , , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
De Gruyter
2025-12-01
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| Serija: | Methods in Microscopy |
| Teme: | |
| Online pristup: | https://doi.org/10.1515/mim-2025-0026 |
| Oznake: |
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