Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED

Simultaneous super-resolution imaging of multiple fluorophores remains a major challenge in STimulated Emission Depletion (STED) microscopy due to spectral overlap of STED-compatible fluorophores. The combination of STED microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) offers a powerfu...

Celý popis

Podrobná bibliografie

Hlavní autoři:	Deschênes Andréanne, Ollier Antoine, Lafontaine Marie, Michaud-Gagnon Albert, Santiague Jeffrey-Gabriel Steavan, Bilodeau Anthony, Gagné Christian, De Koninck Paul, Lavoie-Cardinal Flavie
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	De Gruyter 2025-12-01
Edice:	Methods in Microscopy
Témata:	sted; split-sted multicolor flim phasors
On-line přístup:	https://doi.org/10.1515/mim-2025-0026
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!