Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED

Simultaneous super-resolution imaging of multiple fluorophores remains a major challenge in STimulated Emission Depletion (STED) microscopy due to spectral overlap of STED-compatible fluorophores. The combination of STED microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) offers a powerfu...

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Bibliografiska uppgifter

Huvudupphov:	Deschênes Andréanne, Ollier Antoine, Lafontaine Marie, Michaud-Gagnon Albert, Santiague Jeffrey-Gabriel Steavan, Bilodeau Anthony, Gagné Christian, De Koninck Paul, Lavoie-Cardinal Flavie
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	De Gruyter 2025-12-01
Serie:	Methods in Microscopy
Ämnen:	sted; split-sted multicolor flim phasors
Länkar:	https://doi.org/10.1515/mim-2025-0026
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