NKX2-2 based nuclei sorting on frozen human archival pancreas enables the enrichment of islet endocrine populations for single-nucleus RNA sequencing

Abstract Background Current approaches to profile the single-cell transcriptomics of human pancreatic endocrine cells almost exclusively rely on freshly isolated islets. However, human islets are limited in availability. Furthermore, the extensive processing steps during islet isolation and subseque...

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Κύριοι συγγραφείς:	Gengqiang Xie, Maria Pilar Toledo, Xue Hu, Hyo Jeong Yong, Pamela Sandoval Sanchez, Chengyang Liu, Ali Naji, Jerome Irianto, Yue J. Wang
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	BMC 2024-04-01
Σειρά:	BMC Genomics
Θέματα:	Single-nucleus RNA-seq NKX2-2 Fluorescence-activated nuclei sorting (FANS) Frozen archival human pancreas Islets Endocrine population enrichment
Διαθέσιμο Online:	https://doi.org/10.1186/s12864-024-10335-w
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