NKX2-2 based nuclei sorting on frozen human archival pancreas enables the enrichment of islet endocrine populations for single-nucleus RNA sequencing

Abstract Background Current approaches to profile the single-cell transcriptomics of human pancreatic endocrine cells almost exclusively rely on freshly isolated islets. However, human islets are limited in availability. Furthermore, the extensive processing steps during islet isolation and subseque...

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Detalhes bibliográficos

Principais autores:	Gengqiang Xie, Maria Pilar Toledo, Xue Hu, Hyo Jeong Yong, Pamela Sandoval Sanchez, Chengyang Liu, Ali Naji, Jerome Irianto, Yue J. Wang
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2024-04-01
coleção:	BMC Genomics
Assuntos:	Single-nucleus RNA-seq NKX2-2 Fluorescence-activated nuclei sorting (FANS) Frozen archival human pancreas Islets Endocrine population enrichment
Acesso em linha:	https://doi.org/10.1186/s12864-024-10335-w
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