Reverse Transcriptase Adds Nontemplated Nucleotides to cDNAs During 5′-RACE and Primer Extension
In determining the terminal sequences of the genomic dsRNAs of rotavirus by 5′-rapid amplification of cDNA ends (5′-RACE), it was found that most of the viral cDNAs contained extra nucleotides at their 5′ termini that had not been reported before on any rotavirus sequence. Although the extra nucleot...
Gardado en:
| Principais autores: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Taylor & Francis Group
2001-03-01
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| Series: | BioTechniques |
| Acceso en liña: | https://www.future-science.com/doi/10.2144/01303rr02 |
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