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Reverse Transcriptase Adds Nontemplated Nucleotides to cDNAs During 5′-RACE and Primer Extension

In determining the terminal sequences of the genomic dsRNAs of rotavirus by 5′-rapid amplification of cDNA ends (5′-RACE), it was found that most of the viral cDNAs contained extra nucleotides at their 5′ termini that had not been reported before on any rotavirus sequence. Although the extra nucleot...

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Váldodahkkit: Dayue Chen, John T. Patton
Materiálatiipa: Artigo
Giella:Inglês
Almmustuhtton: Taylor & Francis Group 2001-03-01
Ráidu:BioTechniques
Liŋkkat:https://www.future-science.com/doi/10.2144/01303rr02
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