Reverse Transcriptase Adds Nontemplated Nucleotides to cDNAs During 5′-RACE and Primer Extension
In determining the terminal sequences of the genomic dsRNAs of rotavirus by 5′-rapid amplification of cDNA ends (5′-RACE), it was found that most of the viral cDNAs contained extra nucleotides at their 5′ termini that had not been reported before on any rotavirus sequence. Although the extra nucleot...
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| Principais autores: | , |
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| 格式: | Artigo |
| 语言: | Inglês |
| 出版: |
Taylor & Francis Group
2001-03-01
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| 丛编: | BioTechniques |
| 在线阅读: | https://www.future-science.com/doi/10.2144/01303rr02 |
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