Efficient Long-PCR Site-Specific Mutagenesis of a High GC Template
A long PCR method was developed for the efficient site-specific mutagenesis of herpes simplex virus (HSV-1) DNA fragments with high GC content. In this protocol, a PCR product was partially extended first using a cloned DNA fragment. The final mutagenized fragment was produced after a second extensi...
Gardado en:
| Principais autores: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Taylor & Francis Group
1996-09-01
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| Series: | BioTechniques |
| Acceso en liña: | https://www.future-science.com/doi/10.2144/96213st05 |
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