Efficient Long-PCR Site-Specific Mutagenesis of a High GC Template
A long PCR method was developed for the efficient site-specific mutagenesis of herpes simplex virus (HSV-1) DNA fragments with high GC content. In this protocol, a PCR product was partially extended first using a cloned DNA fragment. The final mutagenized fragment was produced after a second extensi...
Guardat en:
| Autors principals: | , , , |
|---|---|
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Taylor & Francis Group
1996-09-01
|
| Col·lecció: | BioTechniques |
| Accés en línia: | https://www.future-science.com/doi/10.2144/96213st05 |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|