Efficient Long-PCR Site-Specific Mutagenesis of a High GC Template
A long PCR method was developed for the efficient site-specific mutagenesis of herpes simplex virus (HSV-1) DNA fragments with high GC content. In this protocol, a PCR product was partially extended first using a cloned DNA fragment. The final mutagenized fragment was produced after a second extensi...
Zapisane w:
| Główni autorzy: | , , , |
|---|---|
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
Taylor & Francis Group
1996-09-01
|
| Seria: | BioTechniques |
| Dostęp online: | https://www.future-science.com/doi/10.2144/96213st05 |
| Etykiety: |
Nie ma etykietki, Dołącz pierwszą etykiete!
|