Efficient Long-PCR Site-Specific Mutagenesis of a High GC Template
A long PCR method was developed for the efficient site-specific mutagenesis of herpes simplex virus (HSV-1) DNA fragments with high GC content. In this protocol, a PCR product was partially extended first using a cloned DNA fragment. The final mutagenized fragment was produced after a second extensi...
保存先:
| 主要な著者: | , , , |
|---|---|
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Taylor & Francis Group
1996-09-01
|
| シリーズ: | BioTechniques |
| オンライン･アクセス: | https://www.future-science.com/doi/10.2144/96213st05 |
| タグ: |
タグなし, このレコードへの初めてのタグを付けませんか!
|