Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Abstract Single-chain variable fragments (scFvs) have gained increased attention among researchers in both academic and industrial fields owing to simple production in E. coli. The E. coli periplasm has been the site of choice for the expression of scFv molecules due to its oxidizing milieu facilita...

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Detalhes bibliográficos

Principais autores:	Elham Ghamghami, Marjan Abri Aghdam, Mohammad Reza Tohidkia, Asadollah Ahmadikhah, Morteza Khanmohammadi, Tayebeh Mehdipour, Ahad Mokhtarzadeh, Behzad Baradaran
Formato:	Artigo
Idioma:	Inglês
Publicado em:	SpringerOpen 2020-07-01
coleção:	AMB Express
Assuntos:	TES extraction Periplasmic extraction scFv E. coli
Acesso em linha:	http://link.springer.com/article/10.1186/s13568-020-01063-x
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