Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Abstract Single-chain variable fragments (scFvs) have gained increased attention among researchers in both academic and industrial fields owing to simple production in E. coli. The E. coli periplasm has been the site of choice for the expression of scFv molecules due to its oxidizing milieu facilita...

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Autors principals:	Elham Ghamghami, Marjan Abri Aghdam, Mohammad Reza Tohidkia, Asadollah Ahmadikhah, Morteza Khanmohammadi, Tayebeh Mehdipour, Ahad Mokhtarzadeh, Behzad Baradaran
Format:	Artigo
Idioma:	Inglês
Publicat:	SpringerOpen 2020-07-01
Col·lecció:	AMB Express
Matèries:	TES extraction Periplasmic extraction scFv E. coli
Accés en línia:	http://link.springer.com/article/10.1186/s13568-020-01063-x
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