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Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Abstract Single-chain variable fragments (scFvs) have gained increased attention among researchers in both academic and industrial fields owing to simple production in E. coli. The E. coli periplasm has been the site of choice for the expression of scFv molecules due to its oxidizing milieu facilita...

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Principais autores: Elham Ghamghami, Marjan Abri Aghdam, Mohammad Reza Tohidkia, Asadollah Ahmadikhah, Morteza Khanmohammadi, Tayebeh Mehdipour, Ahad Mokhtarzadeh, Behzad Baradaran
פורמט: Artigo
שפה:Inglês
יצא לאור: SpringerOpen 2020-07-01
סדרה:AMB Express
נושאים:
TES extraction
Periplasmic extraction
scFv
E. coli
גישה מקוונת:http://link.springer.com/article/10.1186/s13568-020-01063-x
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