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Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Abstract Single-chain variable fragments (scFvs) have gained increased attention among researchers in both academic and industrial fields owing to simple production in E. coli. The E. coli periplasm has been the site of choice for the expression of scFv molecules due to its oxidizing milieu facilita...

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Bibliografiset tiedot
Päätekijät: Elham Ghamghami, Marjan Abri Aghdam, Mohammad Reza Tohidkia, Asadollah Ahmadikhah, Morteza Khanmohammadi, Tayebeh Mehdipour, Ahad Mokhtarzadeh, Behzad Baradaran
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: SpringerOpen 2020-07-01
Sarja:AMB Express
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Linkit:http://link.springer.com/article/10.1186/s13568-020-01063-x
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