An enzyme-coupled assay for acyl-CoA synthetase.
An enzyme-coupled, colorimetric method for the assay of acyl-CoA synthetase is described. Acyl-CoA formed from fatty acid and CoA by acyl-CoA synthetase was dehydrogenated by acyl-CoA oxidase. Hydrogen peroxide produced was then converted into formaldehyde in the presence of methanol by catalase. Th...
Enregistré dans:
| Auteurs principaux: | , |
|---|---|
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Elsevier
1991-10-01
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| Collection: | Journal of Lipid Research |
| Accès en ligne: | http://www.sciencedirect.com/science/article/pii/S0022227520416566 |
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