An enzyme-coupled assay for acyl-CoA synthetase.
An enzyme-coupled, colorimetric method for the assay of acyl-CoA synthetase is described. Acyl-CoA formed from fatty acid and CoA by acyl-CoA synthetase was dehydrogenated by acyl-CoA oxidase. Hydrogen peroxide produced was then converted into formaldehyde in the presence of methanol by catalase. Th...
保存先:
| 主要な著者: | , |
|---|---|
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Elsevier
1991-10-01
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| シリーズ: | Journal of Lipid Research |
| オンライン･アクセス: | http://www.sciencedirect.com/science/article/pii/S0022227520416566 |
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