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An enzyme-coupled assay for acyl-CoA synthetase.

An enzyme-coupled, colorimetric method for the assay of acyl-CoA synthetase is described. Acyl-CoA formed from fatty acid and CoA by acyl-CoA synthetase was dehydrogenated by acyl-CoA oxidase. Hydrogen peroxide produced was then converted into formaldehyde in the presence of methanol by catalase. Th...

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Autori principali: K Ichihara, Y Shibasaki
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 1991-10-01
Serie:Journal of Lipid Research
Accesso online:http://www.sciencedirect.com/science/article/pii/S0022227520416566
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