Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity

Por Basila, Megan, Kelley, Melissa L., Smith, Anja van Brabant
Publicado no PLoS One (2017)

Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference

Por Barrangou, Rodolphe, Birmingham, Amanda, Wiemann, Stefan, Beijersbergen, Roderick L., Hornung, Veit, Smith, Anja van Brabant
Publicado no Nucleic Acids Res (2015)

Lactobacillus gasseri CRISPR-Cas9 characterization In Vitro reveals a flexible mode of protospacer-adjacent motif recognition

Por Anderson, Emily M., McClelland, Shawn, Maksimova, Elena, Strezoska, Žaklina, Basila, Megan, Briner, Alexandra E., Barrangou, Rodolphe, Smith, Anja van Brabant
Publicado no PLoS One (2018)

Amide linkages mimic phosphates in RNA interactions with proteins and are well tolerated in the guide strand of short interfering RNAs

Por Mutisya, Daniel, Hardcastle, Travis, Cheruiyot, Samwel K., Pallan, Pradeep S., Kennedy, Scott D., Egli, Martin, Kelley, Melissa L., Smith, Anja van Brabant, Rozners, Eriks
Publicado no Nucleic Acids Res (2017)