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Local and Systemic Neutralizing Antibody Responses Induced by Intranasal Immunization with the Nontoxic Binding Domain of Toxin A from Clostridium difficile

Fourteen of the 38 C-terminal repeats from Clostridium difficile toxin A (14CDTA) were cloned and expressed either with an N-terminal polyhistidine tag (14CDTA-HIS) or fused to the nontoxic binding domain from tetanus toxin (14CDTA-TETC). The recombinant proteins were successfully purified by bovine...

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Autors principals: Ward, Stephen J., Douce, Gill, Dougan, Gordon, Wren, Brendan W.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC96861/
https://ncbi.nlm.nih.gov/pubmed/10496886
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