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DNA sequences from multiple amplifications reveal artifacts induced by cytosine deamination in ancient DNA

We show that DNA molecules amplified by PCR from DNA extracted from animal bones and teeth that vary in age between 25 000 and over 50 000 years carry C→T and G→A substitutions. These substitutions can reach high proportions among the molecules amplified and are due to the occurrence of modified deo...

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Библиографические подробности
Главные авторы: Hofreiter, Michael, Jaenicke, Viviane, Serre, David, Haeseler, Arndt von, Pääbo, Svante
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 2001
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC96698/
https://ncbi.nlm.nih.gov/pubmed/11726688
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