Properties and Mutation Analysis of the CelK Cellulose-Binding Domain from the Clostridium thermocellum Cellulosome

The family IV cellulose-binding domain of Clostridium thermocellum CelK (CBD(CelK)) was expressed in Escherichia coli and purified. It binds to acid-swollen cellulose (ASC) and bacterial microcrystalline cellulose (BMCC) with capacities of 16.03 and 3.95 μmol/g of cellulose and relative affinities (...

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Bibliographic Details
Main Authors: Kataeva, Irina A., Seidel, Ronald D., Li, Xin-Liang, Ljungdahl, Lars G.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2001
Subjects:
Enzymes and Proteins
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC95039/
https://ncbi.nlm.nih.gov/pubmed/11160085
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.5.1552-1559.2001
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