Properties and Mutation Analysis of the CelK Cellulose-Binding Domain from the Clostridium thermocellum Cellulosome

The family IV cellulose-binding domain of Clostridium thermocellum CelK (CBD(CelK)) was expressed in Escherichia coli and purified. It binds to acid-swollen cellulose (ASC) and bacterial microcrystalline cellulose (BMCC) with capacities of 16.03 and 3.95 μmol/g of cellulose and relative affinities (...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Kataeva, Irina A., Seidel, Ronald D., Li, Xin-Liang, Ljungdahl, Lars G.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Enzymes and Proteins
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC95039/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11160085/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.183.5.1552-1559.2001
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