Cloning, DNA sequencing, and expression of the gene encoding Clostridium thermocellum cellulase CelJ, the largest catalytic component of the cellulosome.

The Clostridium thermocellum F1 celJ gene, encoding endoglucanase J (CelJ), consists of an open reading frame (ORF) of 4,803 nucleotides and encodes a protein of 1,601 amino acids with a molecular weight of 178,055. The ORF was confirmed as celJ by comparison with the N-terminal sequence of a trunca...

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Bibliographic Details
Main Authors: Ahsan, M M, Kimura, T, Karita, S, Sakka, K, Ohmiya, K
Format: Artigo
Language:Inglês
Published: 1996
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC178413/
https://ncbi.nlm.nih.gov/pubmed/8824619
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