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Systematic Identification of Selective Essential Genes in Helicobacter pylori by Genome Prioritization and Allelic Replacement Mutagenesis

A comparative genomic approach was used to identify Helicobacter pylori 26695 open reading frames (ORFs) which are conserved in H. pylori J99 but highly diverged in other eubacteria. A survey of selected pathways of central intermediary metabolism was also carried out, and genes with a potentially s...

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Udgivet i:J Bacteriol
Principais autores: Chalker, Alison F., Minehart, Heather W., Hughes, Nicky J., Koretke, Kristin K., Lonetto, Michael A., Brinkman, Kerry K., Warren, Patrick V., Lupas, Andrei, Stanhope, Michael J., Brown, James R., Hoffman, Paul S.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2001
Fag:
Genetics and Molecular Biology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94999/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11157938/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.183.4.1259-1268.2001
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