Systematic Identification of Selective Essential Genes in Helicobacter pylori by Genome Prioritization and Allelic Replacement Mutagenesis

A comparative genomic approach was used to identify Helicobacter pylori 26695 open reading frames (ORFs) which are conserved in H. pylori J99 but highly diverged in other eubacteria. A survey of selected pathways of central intermediary metabolism was also carried out, and genes with a potentially s...

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Detalhes bibliográficos
Main Authors: Chalker, Alison F., Minehart, Heather W., Hughes, Nicky J., Koretke, Kristin K., Lonetto, Michael A., Brinkman, Kerry K., Warren, Patrick V., Lupas, Andrei, Stanhope, Michael J., Brown, James R., Hoffman, Paul S.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC94999/
https://ncbi.nlm.nih.gov/pubmed/11157938
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.4.1259-1268.2001
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