Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange.

Isogenic urease-negative mutants of Helicobacter pylori were constructed by allelic replacement. A region of cloned H. pylori DNA containing the structural urease genes (ureA and ureB) was disrupted by insertion of a mini-Tn3-Km transposon. Electrotransformation of H. pylori cells with kanamycin-ure...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Ferrero, R L, Cussac, V, Courcoux, P, Labigne, A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1992
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206198/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1320607/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.13.4212-4217.1992
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