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Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange.

Isogenic urease-negative mutants of Helicobacter pylori were constructed by allelic replacement. A region of cloned H. pylori DNA containing the structural urease genes (ureA and ureB) was disrupted by insertion of a mini-Tn3-Km transposon. Electrotransformation of H. pylori cells with kanamycin-ure...

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Autori principali: Ferrero, R L, Cussac, V, Courcoux, P, Labigne, A
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1992
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC206198/
https://ncbi.nlm.nih.gov/pubmed/1320607
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