Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange.
Isogenic urease-negative mutants of Helicobacter pylori were constructed by allelic replacement. A region of cloned H. pylori DNA containing the structural urease genes (ureA and ureB) was disrupted by insertion of a mini-Tn3-Km transposon. Electrotransformation of H. pylori cells with kanamycin-ure...
Zapisane w:
| Wydane w: | J Bacteriol |
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| Główni autorzy: | , , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
American Society for Microbiology (ASM)
1992
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206198/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1320607/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.13.4212-4217.1992 |
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