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Characterization of the Streptococcal C5a Peptidase Using a C5a-Green Fluorescent Protein Fusion Protein Substrate
A glutathione-S-transferase (GST)–C5a–green fluorescent protein (GFP) fusion protein was designed for use as a substrate for the streptococcal C5a peptidase (SCPA). The substrate was immobilized on a glutathione-Sepharose affinity matrix and used to measure wild-type SCPA activity in the range of 0....
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Main Authors: | , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
American Society for Microbiology
2000
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC94514/ https://ncbi.nlm.nih.gov/pubmed/10809707 |
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