Characterization of the Streptococcal C5a Peptidase Using a C5a-Green Fluorescent Protein Fusion Protein Substrate

A glutathione-S-transferase (GST)–C5a–green fluorescent protein (GFP) fusion protein was designed for use as a substrate for the streptococcal C5a peptidase (SCPA). The substrate was immobilized on a glutathione-Sepharose affinity matrix and used to measure wild-type SCPA activity in the range of 0....

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Pubblicato in:	J Bacteriol
Autori principali:	Stafslien, D. K., Cleary, P. P.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2000
Soggetti:	Enzymes and Proteins
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94514/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10809707/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.11.3254-3258.2000
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