The pur7 Gene from the Puromycin Biosynthetic pur Cluster of Streptomyces alboniger Encodes a Nudix Hydrolase

Pur7 is the product of a gene from the puromycin biosynthetic pur cluster of Streptomyces alboniger. It was expressed in Escherichia coli as a recombinant protein fused to a His tag and then was highly purified through a Ni(2+) column. It showed a 3′-amino-3′-dATP pyrophosphohydrolase (nudix) activi...

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Hlavní autoři: Espinosa, J. C., Tercero, J. A., Rubio, M. A., Jiménez, A.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 1999
Témata:
Enzymes and Proteins
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC93979/
https://ncbi.nlm.nih.gov/pubmed/10438762
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