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Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.

The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromyc...

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Bibliografiske detaljer
Main Authors: Vara, J A, Portela, A, Ortín, J, Jiménez, A
Format: Artigo
Sprog:Inglês
Udgivet: 1986
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC311469/
https://ncbi.nlm.nih.gov/pubmed/3714487
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