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Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.
The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromyc...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1986
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311469/ https://ncbi.nlm.nih.gov/pubmed/3714487 |
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