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Saturation Mutagenesis of the TATA Box and Upstream Activator Sequence in the Haloarchaeal bop Gene Promoter

Degenerate oligonucleotides were used to randomize 21 bp of the 53-bp minimal bop promoter in three 7-bp segments, including the putative TATA box and the upstream activator sequence (UAS). The mutagenized bop promoter and the wild-type structural gene and transcriptional terminator were inserted in...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Baliga, Nitin S., DasSarma, Shiladitya
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1999
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93679/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10198017/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.8.2513-2518.1999
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