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Saturation Mutagenesis of the TATA Box and Upstream Activator Sequence in the Haloarchaeal bop Gene Promoter

Degenerate oligonucleotides were used to randomize 21 bp of the 53-bp minimal bop promoter in three 7-bp segments, including the putative TATA box and the upstream activator sequence (UAS). The mutagenized bop promoter and the wild-type structural gene and transcriptional terminator were inserted in...

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Detalles Bibliográficos
Main Authors: Baliga, Nitin S., DasSarma, Shiladitya
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 1999
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC93679/
https://ncbi.nlm.nih.gov/pubmed/10198017
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