Gene Targeting in Penicillium chrysogenum: Disruption of the lys2 Gene Leads to Penicillin Overproduction

Two strategies have been used for targeted integration at the lys2 locus of Penicillium chrysogenum. In the first strategy the disruption of lys2 was obtained by a single crossing over between the endogenous lys2 and a fragment of the same gene located in an integrative plasmid. lys2-disrupted mutan...

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Λεπτομέρειες βιβλιογραφικής εγγραφής

Εκδόθηκε σε:	J Bacteriol
Κύριοι συγγραφείς:	Casqueiro, Javier, Gutiérrez, Santiago, Bañuelos, Oscar, Hijarrubia, Maria Jose, Martín, Juan Francisco
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	American Society for Microbiology (ASM) 1999
Θέματα:	Eukaryotic Cells
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93495/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9973344/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.4.1181-1188.1999
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