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Direct Detection of 16S rRNA in Soil Extracts by Using Oligonucleotide Microarrays
We report on the development and validation of a simple microarray method for the direct detection of intact 16S rRNA from unpurified soil extracts. Total RNAs from Geobacter chapellei and Desulfovibrio desulfuricans were hybridized to an oligonucleotide array consisting of universal and species-spe...
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| Main Authors: | , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology
2001
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC93223/ https://ncbi.nlm.nih.gov/pubmed/11571176 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.10.4708-4716.2001 |
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