Direct Detection of 16S rRNA in Soil Extracts by Using Oligonucleotide Microarrays

We report on the development and validation of a simple microarray method for the direct detection of intact 16S rRNA from unpurified soil extracts. Total RNAs from Geobacter chapellei and Desulfovibrio desulfuricans were hybridized to an oligonucleotide array consisting of universal and species-spe...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Small, Jack, Call, Douglas R., Brockman, Fred J., Straub, Timothy M., Chandler, Darrell P.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2001
Sujets:	Methods
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93223/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11571176/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.10.4708-4716.2001
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