Direct Detection of 16S rRNA in Soil Extracts by Using Oligonucleotide Microarrays

We report on the development and validation of a simple microarray method for the direct detection of intact 16S rRNA from unpurified soil extracts. Total RNAs from Geobacter chapellei and Desulfovibrio desulfuricans were hybridized to an oligonucleotide array consisting of universal and species-spe...

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Detalhes bibliográficos
Main Authors: Small, Jack, Call, Douglas R., Brockman, Fred J., Straub, Timothy M., Chandler, Darrell P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC93223/
https://ncbi.nlm.nih.gov/pubmed/11571176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.10.4708-4716.2001
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