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Direct Detection of 16S rRNA in Soil Extracts by Using Oligonucleotide Microarrays
We report on the development and validation of a simple microarray method for the direct detection of intact 16S rRNA from unpurified soil extracts. Total RNAs from Geobacter chapellei and Desulfovibrio desulfuricans were hybridized to an oligonucleotide array consisting of universal and species-spe...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology
2001
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC93223/ https://ncbi.nlm.nih.gov/pubmed/11571176 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.10.4708-4716.2001 |
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