DNA from Uncultured Organisms as a Source of 2,5-Diketo-d-Gluconic Acid Reductases

Total DNA of a population of uncultured organisms was extracted from soil samples, and by using PCR methods, the genes encoding two different 2,5-diketo-d-gluconic acid reductases (DKGRs) were recovered. Degenerate PCR primers based on published sequence information gave internal gene fragments homo...

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Bibliographic Details
Main Authors: Eschenfeldt, William H., Stols, Lucy, Rosenbaum, Helga, Khambatta, Zubin S., Quaite-Randall, Elsie, Wu, Shan, Kilgore, Donna C., Trent, Jonathan D., Donnelly, Mark I.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2001
Subjects:
Enzymology and Protein Engineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC93149/
https://ncbi.nlm.nih.gov/pubmed/11526025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.9.4206-4214.2001
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