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Optimization of Annealing Temperature To Reduce Bias Caused by a Primer Mismatch in Multitemplate PCR

To reduce PCR bias derived from a primer mismatch, the effect of the annealing temperature on the product ratio was investigated by denaturing gradient gel electrophoresis analysis of PCR products from a mixture of perfect-match and one-mismatch templates. These templates were generated by PCR from...

詳細記述

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書誌詳細
主要な著者: Ishii, Kousuke, Fukui, Manabu
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 2001
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC93085/
https://ncbi.nlm.nih.gov/pubmed/11472961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.8.3753-3755.2001
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