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Optimization of Annealing Temperature To Reduce Bias Caused by a Primer Mismatch in Multitemplate PCR
To reduce PCR bias derived from a primer mismatch, the effect of the annealing temperature on the product ratio was investigated by denaturing gradient gel electrophoresis analysis of PCR products from a mixture of perfect-match and one-mismatch templates. These templates were generated by PCR from...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC93085/ https://ncbi.nlm.nih.gov/pubmed/11472961 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.8.3753-3755.2001 |
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