Secretion of Active Recombinant Human Tissue Plasminogen Activator Derivatives in Escherichia coli
The DNA fragment coding for kringle 2 plus serine protease domains (K2S) of tissue plasminogen activator (tPA) was inserted into a phagemid vector, pComb3HSS. In the recombinant vector, pComb3H-K2S, the K2S gene was fused to gpIII of ΦM13 and linked to the OmpA signal sequence. The resulting gene, r...
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| 出版年: | Appl Environ Microbiol |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology (ASM)
2001
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92921/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11375177/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.6.2657-2664.2001 |
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