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Secretion of Active Recombinant Human Tissue Plasminogen Activator Derivatives in Escherichia coli

The DNA fragment coding for kringle 2 plus serine protease domains (K2S) of tissue plasminogen activator (tPA) was inserted into a phagemid vector, pComb3HSS. In the recombinant vector, pComb3H-K2S, the K2S gene was fused to gpIII of ΦM13 and linked to the OmpA signal sequence. The resulting gene, r...

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Udgivet i:Appl Environ Microbiol
Principais autores: Manosroi, Jiradej, Tayapiwatana, Chatchai, Götz, Friedrich, Werner, Rolf G., Manosroi, Aranya
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2001
Fag:
Enzymology and Protein Engineering
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92921/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11375177/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.6.2657-2664.2001
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