Molecular Characterization of a Theta Replication Plasmid and Its Use for Development of a Two-Component Food-Grade Cloning System for Lactococcus lactis

pCD4, a small, highly stable theta-replicating lactococcal plasmid, was used to develop a food-grade cloning system. Sequence analysis revealed five open reading frames and two putative cis-acting regions. None appears to code for undesirable phenotypes with regard to food applications. Functional a...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Émond, Éric, Lavallée, Richard, Drolet, Geneviève, Moineau, Sylvain, LaPointe, Gisèle
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2001
Schlagworte:	Food Microbiology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92788/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11282624/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.4.1700-1709.2001
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