Development and Application of a Most-Probable-Number–PCR Assay To Quantify Flagellate Populations in Soil Samples

This paper reports on the first successful molecular detection and quantification of soil protozoa. Quantification of heterotrophic flagellates and naked amoebae in soil has traditionally relied on dilution culturing techniques, followed by most-probable-number (MPN) calculations. Such methods are b...

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Bibliographic Details
Main Authors: Fredslund, Line, Ekelund, Flemming, Jacobsen, Carsten Suhr, Johnsen, Kaare
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2001
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC92777/
https://ncbi.nlm.nih.gov/pubmed/11282613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.4.1613-1618.2001
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